Review:
Digital Pcr
overall review score: 4.5
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score is between 0 and 5
Digital PCR (dPCR) is a modern technique used for precise quantification of nucleic acids by digitally counting the number of DNA or RNA molecules in a sample.
Key Features
- High sensitivity and accuracy
- Absolute quantification without the need for standard curves
- Digital partitioning of the sample into thousands of individual reactions
Pros
- Highly accurate quantification of nucleic acids
- Lower limit of detection compared to traditional PCR techniques
- Less affected by inhibitors in the sample
Cons
- Higher cost compared to traditional PCR methods
- Limited throughput for high volume samples
- Requires specialized equipment and expertise